ER-tethered RNA-binding protein controls NADPH oxidase translation for hydrogen peroxide homeostasis.

ABSTRACT: Hydrogen peroxide (H₂O₂) functions as a signaling molecule in diverse cellular processes. While cells have evolved the capability to detect and manage changes in H₂O₂ levels, the mechanisms regulating key H₂O₂-producing enzymes to maintain optimal levels, especially in pancreatic beta cells with notably weak antioxidative defense, remain unclear. We found that the protein EI24 responds to changes in H₂O₂ concentration and regulates the production of H₂O₂ by controlling the translation of NOX4, an enzyme that is constitutively active, achieved by recruiting an RNA-binding protein, RTRAF, to the 3'-UTR of Nox4. Depleting EI24 results in RTRAF relocating into the nucleus, releasing th

SUBMITTER: Pei X 

PROVIDER: S-EPMC10963860 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature