MicroRNA-34a-5p: A pivotal therapeutic target in gallbladder cancer.

ABSTRACT: Gallbladder cancer incidence has been increasing globally, and it remains challenging to expect long prognosis with the current systemic chemotherapy. We identified a novel nucleic acid-mediated therapeutic target against gallbladder cancer by using innovative organoid-based gallbladder cancer models generated from Kras^LSL-G12D/+; Trp53^f/f mice. Using comprehensive microRNA expression analyses and a bioinformatics approach, we identified significant microRNA-34a-5p downregulation in both murine gallbladder cancer organoids and resected human gallbladder cancer specimens. In three different human gallbladder cancer cell lines, forced microRNA-34a-5p expression inhibited cell proliferation and induced cell-cycle arrest at the G1 phase by suppressing direct

SUBMITTER: Oda T 

PROVIDER: S-EPMC10963938 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature