IL-10 dampens antitumor immunity and promotes liver metastasis via PD-L1 induction.

ABSTRACT:

Background & aims

The liver is one of the organs most commonly affected by metastasis. The presence of liver metastases has been reported to be responsible for an immunosuppressive microenvironment and diminished immunotherapy efficacy. Herein, we aimed to investigate the role of IL-10 in liver metastasis and to determine how its modulation could affect the efficacy of immunotherapy in vivo.

Methods

To induce spontaneous or forced liver metastasis in mice, murine cancer cells (MC38) or colon tumor organoids were injected into the cecum or the spleen, respectively. Mice with complete and cell type-specific deletion of IL-10 and IL-10 receptor alpha were used to identify the source and the target of IL-10 during metastasis formation. Programmed death ligand 1 (PD-L1)-deficient

SUBMITTER: Shiri AM 

PROVIDER: S-EPMC10964083 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature