Engineering the carbon and redox metabolism of Paenibacillus polymyxa for efficient isobutanol production.

ABSTRACT: Paenibacillus polymyxa is a non-pathogenic, Gram-positive bacterium endowed with a rich and versatile metabolism. However interesting, this bacterium has been seldom used for bioproduction thus far. In this study, we engineered P. polymyxa for isobutanol production, a relevant bulk chemical and next-generation biofuel. A CRISPR-Cas9-based genome editing tool facilitated the chromosomal integration of a synthetic operon to establish isobutanol production. The 2,3-butanediol biosynthesis pathway, leading to the main fermentation product of P. polymyxa, was eliminated. A mutant strain harbouring the synthetic isobutanol operon (kdcA from Lactococcus lactis, and the native ilvC, ilvD and adh genes) produced 1 g L^-1 isobutanol under microaerobic conditions. Improving NADPH regenerati

SUBMITTER: Meliawati M 

PROVIDER: S-EPMC10964175 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature