Liver sinusoidal cells eliminate blood-borne phage K1F.

ABSTRACT: Phage treatment has regained attention due to an increase in multiresistant bacteria. For phage therapy to be successful, phages must reach their target bacteria in sufficiently high numbers. Blood-borne phages are believed to be captured by macrophages in the liver and spleen. Since liver sinusoids also consist of specialized scavenger liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs), this study investigated the contribution of both cell types in the elimination of Escherichia coli phage K1Fg10b::gfp (K1F^gfp) in mice. Circulatory half-life, organ, and hepatocellular distribution of K1F^gfp were determined following intravenous administration. Internalization of K1F^gfp and effects of phage opsonization on uptake were explored using p

SUBMITTER: Sanchez Romano J 

PROVIDER: S-EPMC10964407 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature