Matrix-free human pluripotent stem cell manufacturing by seed train approach and intermediate cryopreservation.

ABSTRACT:

Background

Human pluripotent stem cells (hPSCs) have an enormous therapeutic potential, but large quantities of cells will need to be supplied by reliable, economically viable production processes. The suspension culture (three-dimensional; 3D) of hPSCs in stirred tank bioreactors (STBRs) has enormous potential for fuelling these cell demands. In this study, the efficient long-term matrix-free suspension culture of hPSC aggregates is shown.

Methods and results

STBR-controlled, chemical aggregate dissociation and optimized passage duration of 3 or 4 days promotes exponential hPSC proliferation, process efficiency and upscaling by a seed train approach. Intermediate high-density cryopreservation of suspension-derived hPSCs followed by direct STBR inoculation enabled complete o

SUBMITTER: Ullmann K 

PROVIDER: S-EPMC10964510 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature