Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples.

ABSTRACT:

Background

Sequencing variable regions of the 16S rRNA gene (≃300 bp) with Illumina technology is commonly used to study the composition of human microbiota. Unfortunately, short reads are unable to differentiate between highly similar species. Considering that species from the same genus can be associated with health or disease it is important to identify them at the lowest possible taxonomic rank. Third-generation sequencing platforms such as PacBio SMRT, increase read lengths allowing to sequence the whole gene with the maximum taxonomic resolution. Despite its potential, full length 16S rRNA gene sequencing is not widely used yet. The aim of the current study was to compare the sequencing output and taxonomic annotation performance of the two approaches (Illumina short read seq

SUBMITTER: Buetas E 

PROVIDER: S-EPMC10964587 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature