Dataset Information

Single-cell RNA sequencing reveals recruitment of the M2-like CCL8^high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy.

ABSTRACT:

Background

Tumor-associated macrophages (TAMs) play a pivotal role in reshaping the tumor microenvironment following radiotherapy. The mechanisms underlying this reprogramming process remain to be elucidated.

Methods

Subcutaneous Lewis lung carcinoma (LLC) murine model was treated with hypofrationated radiotherapy (8 Gy × 3F). Single-cell RNA sequencing was utilized to identify subclusters and functions of TAMs. Multiplex assay and enzyme-linked immunosorbent assay (ELISA) were employed to measure serum chemokine levels. Bindarit was used to inhibit CCL8, CCL7, and CCL2. The infiltration of TAMs after combination treatment with hypofractionated radiotherapy and Bindarit was quantified with flow cytometry, while the influx of CD206 and CCL8 was assessed by immunostaining.

SUBMITTER: Yang H

PROVIDER: S-EPMC10964592 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Dataset Information

Single-cell RNA sequencing reveals recruitment of the M2-like CCL8^high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy.

Background

Methods

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Single-cell RNA sequencing reveals recruitment of the M2-like CCL8high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy.

Background

Methods

OmicsDI is part of the ELIXIR infrastructure

Tweets

Single-cell RNA sequencing reveals recruitment of the M2-like CCL8^high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy.