Coupling of nanostraws with diverse physicochemical perforation strategies for intracellular DNA delivery.

ABSTRACT: Effective intracellular DNA transfection is imperative for cell-based therapy and gene therapy. Conventional gene transfection methods, including biochemical carriers, physical electroporation and microinjection, face challenges such as cell type dependency, low efficiency, safety concerns, and technical complexity. Nanoneedle arrays have emerged as a promising avenue for improving cellular nucleic acid delivery through direct penetration of the cell membrane, bypassing endocytosis and endosome escape processes. Nanostraws (NS), characterized by their hollow tubular structure, offer the advantage of flexible solution delivery compared to solid nanoneedles. However, NS struggle to stably self-penetrate the cell membrane, resulting in limited delivery efficiency. Coupling with extra physioch

SUBMITTER: Jiang J 

PROVIDER: S-EPMC10964692 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature