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In situ imaging of mRNA transcripts based on split-aptamer and split protein in living cells.

ABSTRACT: Messenger RNA (mRNA) is an essential component of cell development and growth. However, the detection of endogenous mRNA in living cells is currently limited. To address this issue, we have developed a novel strategy that comprises split-aptamer and split fluorescent protein dual-color, "turn-on" probes that specifically target mRNA with complementary sequences. Our split-aptamer and split-protein-initiated fluorescence complementation (sAPiFC) approach for live-cell imaging has demonstrated selectivity, stability, and capability for targeting various mRNAs.

SUBMITTER: Peng Y

PROVIDER: S-EPMC10964932 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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<i>In situ</i> imaging of mRNA transcripts based on split-aptamer and split protein in living cells.

Peng Yan Y Li Mengqi M Gong Fuyu F Liu Xiaofei X Xiong Jirou J Wang Keran K

RSC advances 20240326 15

Messenger RNA (mRNA) is an essential component of cell development and growth. However, the detection of endogenous mRNA in living cells is currently limited. To address this issue, we have developed a novel strategy that comprises split-aptamer and split fluorescent protein dual-color, "turn-on" probes that specifically target mRNA with complementary sequences. Our split-aptamer and split-protein-initiated fluorescence complementation (sAPiFC) approach for live-cell imaging has demonstrated sel ...[more]

PMID: 38544938

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