Spindle checkpoint activation by fungal orthologs of the S. cerevisiae Mps1 kinase.

ABSTRACT: There is an ongoing need for antifungal agents to treat humans. Identification of new antifungal agents can be based on screening compounds using whole cell assays. Screening compounds that target a particular molecule is possible in budding yeast wherein sophisticated strain engineering allows for controlled expression of endogenous or heterologous genes. We have considered the yeast Mps1 protein kinase as a reasonable target for antifungal agents because mutant or druggable forms of the protein, upon inactivation, cause rapid loss of cell viability. Furthermore, extensive analysis of the Mps1 in budding yeast has offered potential tactics for identifying inhibitors of its enzymatic activity. One such tactic is based on the finding that overexpression of Mps1 leads to cell cycle arrest vi

SUBMITTER: Fabritius A 

PROVIDER: S-EPMC10965065 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature