MicroBundleCompute: Automated segmentation, tracking, and analysis of subdomain deformation in cardiac microbundles.

ABSTRACT: Advancing human induced pluripotent stem cell derived cardiomyocyte (hiPSC-CM) technology will lead to significant progress ranging from disease modeling, to drug discovery, to regenerative tissue engineering. Yet, alongside these potential opportunities comes a critical challenge: attaining mature hiPSC-CM tissues. At present, there are multiple techniques to promote maturity of hiPSC-CMs including physical platforms and cell culture protocols. However, when it comes to making quantitative comparisons of functional behavior, there are limited options for reliably and reproducibly computing functional metrics that are suitable for direct cross-system comparison. In addition, the current standard functional metrics obtained from time-lapse images of cardiac microbundle contraction reported

SUBMITTER: Kobeissi H 

PROVIDER: S-EPMC10965069 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature