Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose.

ABSTRACT: To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding of adaptation processes primed by rational metabolic engineering remain scarce. The industrial workhorse Pseudomonas putida was engineered to utilize the non-native sugar D-xylose, but its assimilation into the bacterial biochemical network via the exogenous xylose isomerase pathway remained unresolved. Here, we elucidate the xylose metabolism and establish a foundation for further engineering followed by ALE. First, native glycolysis is derepressed by deleting the local transcriptional regulator gene hexR. We then enhance the pentose phosphate pat

SUBMITTER: Dvorak P 

PROVIDER: S-EPMC10965963 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature