Nanopore-based metagenomics analysis reveals microbial presence in amniotic fluid: A prospective study.

ABSTRACT:

Background

Current research on amniotic fluid (AF) microbiota yields contradictory data, necessitating an accurate, comprehensive, and scientifically rigorous evaluation.

Objective

This study aimed to characterise the microbial features of AF and explore the correlation between microbial information and clinical parameters.

Methods

76 AF samples were collected in this prospective cohort study. Fourteen samples were utilised to establish the nanopore metagenomic sequencing methodology, whereas the remaining 62 samples underwent a final statistical analysis along with clinical information. Negative controls included the operating room environment (OE), surgical instruments (SI), and laboratory experimental processes (EP) to elucidate the background contamination at each

SUBMITTER: Zhong L 

PROVIDER: S-EPMC10966708 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature