Dataset Information

Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells.

ABSTRACT: Recent studies have demonstrated that immune-related recombinant proteins can enhance immune function, increasing host survival against infectious diseases in salmonids. This research evaluated inclusion bodies (IBs) of antimicrobial peptides (CAMP^IB and HAMP^IB) and a cytokine (IL1β^IB and TNFα^IB) as potential immunostimulants in farmed salmonids. For this purpose, we produced five IBs (including iRFP^IB as a control), and we evaluated their ability to modulate immune marker gene expression of three IBs in the RTS11 cell line by RT-qPCR. Additionally, we characterized the scale-up of IBs production by comparing two different scale systems. The results showed that CAMP^IB can increase the upregulation of tnfα, il1β, il8, and il10, HAMP^IB significantly increases the upregulation of tnfα, inos, and il10, and IL1β^IB significantly upregulated the expression of tnfα, il1β, and cox2. A comparison of IL1β^IB production showed that the yield was greater in shake flasks than in bioreactors (39 ± 1.15 mg/L and 14.5 ± 4.08 mg/L), and larger nanoparticles were produced in shake flasks (540 ± 129 nm and 427 ± 134 nm, p < 0.0001, respectively). However, compared with its shake flask counterpart, the IL1β^IB produced in a bioreactor has an increased immunomodulatory ability. Further studies are needed to understand the immune response pathways activated by IBs and the optimal production conditions in bioreactors, such as a defined medium, fed-batch production, and mechanical bacterial lysis, to increase yield.

SUBMITTER: Torrealba D

PROVIDER: S-EPMC10967322 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells.

Torrealba Débora D López Daniela D Zelada Patricio P Salinas-Parra Nicolás N Valenzuela-Avilés Paula P Garcia-Fruitós Elena E Arís Anna A Mercado Luis L Altamirano Claudia C Gallardo-Matus José J

Animals : an open access journal from MDPI 20240309 6

Recent studies have demonstrated that immune-related recombinant proteins can enhance immune function, increasing host survival against infectious diseases in salmonids. This research evaluated inclusion bodies (IBs) of antimicrobial peptides (CAMPIB and HAMPIB) and a cytokine (IL1βIB and TNFαIB) as potential immunostimulants in farmed salmonids. For this purpose, we produced five IBs (including iRFPIB as a control), and we evaluated their a ...[more]

PMID: 38539943

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Project description:Antimicrobial resistance (AMR) is a significant problem in health care, animal health, and food safety. To limit AMR, there is a need for alternatives to antibiotics to enhance disease resistance and support judicious antibiotic usage in animals and humans. Immunomodulation is a promising strategy to enhance disease resistance without antibiotics in food animals. One rapidly evolving field of immunomodulation is innate memory in which innate immune cells undergo epigenetic changes of chromatin remodeling and metabolic reprogramming upon a priming event that results in either enhanced or suppressed responsiveness to secondary stimuli (training or tolerance, respectively). Exposure to live agents such as bacille Calmette-Guerin (BCG) or microbe-derived products such as LPS or yeast cell wall ß-glucans can reprogram or "train" the innate immune system. Over the last decade, significant advancements increased our understanding of innate training in humans and rodent models, and strategies are being developed to specifically target or regulate innate memory. In veterinary species, the concept of enhancing the innate immune system is not new; however, there are few available studies which have purposefully investigated innate training as it has been defined in human literature. The development of targeted approaches to engage innate training in food animals, with the practical goal of enhancing the capacity to limit disease without the use of antibiotics, is an area which deserves attention. In this review, we provide an overview of innate immunomodulation and memory, and the mechanisms which regulate this long-term functional reprogramming in other animals (e.g., humans, rodents). We focus on studies describing innate training, or similar phenomenon (often referred to as heterologous or non-specific protection), in cattle, sheep, goats, swine, poultry, and fish species; and discuss the potential benefits and shortcomings of engaging innate training for enhancing disease resistance.

Dataset Information

Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells.

Publications

Immunomodulation Evidence of Nanostructured Recombinant Proteins in Salmonid Cells.

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