Changes in Gravitaxis and Gene-Expression in an Euglena gracilis Culture over Time.

ABSTRACT: Age-dependent changes in the transcription levels of 5-day-old Euglena gracilis cells, which showed positive gravitaxis, 6-day-old cells without gravitactic orientation, and older cells (9- and 11-day-old, which displayed a precise negative gravitaxis) were determined through microarray analysis. Hierarchical clustering of four independent cell cultures revealed pronounced similarities in transcription levels at the same culture age, which proves the reproducibility of the cultivation method. Employing the non-oriented cells from the 6-day-old culture as a reference, about 2779 transcripts were found to be differentially expressed. While positively gravitactic cells (5-day-old culture) showed only minor differences in gene expression compared to the 6-day reference, pronounced chang

SUBMITTER: Kruger J 

PROVIDER: S-EPMC10967724 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature