Real-Time On-Site Monitoring of Viruses in Wastewater Using Nanotrap^® Particles and RICCA Technologies.

ABSTRACT: Wastewater-based epidemiology (WBE) is an effective and efficient tool for the early detection of infectious disease outbreaks in a community. However, currently available methods are laborious, costly, and time-consuming due to the low concentration of viruses and the presence of matrix chemicals in wastewater that may interfere with molecular analyses. In the present study, we designed a highly sensitive "Quick Poop (wastewater with fecal waste) Sensor" (termed, QPsor) using a joint approach of Nanotrap microbiome particles and RICCA (RNA Isothermal Co-Assisted and Coupled Amplification). Using QPsor, the WBE study showed a strong correlation with standard PEG concentrations and the qPCR technique. Using a closed format for a paper-based lateral flow assay, we were able to demonstrate th

SUBMITTER: Sharma V 

PROVIDER: S-EPMC10967975 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature