Switching Separation Migration Order by Switching Electrokinetic Regime in Electrokinetic Microsystems.

ABSTRACT: Analyte migration order is a major aspect in all migration-based analytical separations methods. Presented here is the manipulation of the migration order of microparticles in an insulator-based electrokinetic separation. Three distinct particle mixtures were studied: a binary mixture of particles with similar electrical charge and different sizes, and two tertiary mixtures of particles of distinct sizes. Each one of the particle mixtures was separated twice, the first separation was performed under low voltage (linear electrokinetic regime) and the second separation was performed under high voltage (nonlinear electrokinetic regime). Linear electrophoresis, which discriminates particles by charge, is the dominant electrokinetic effect in the linear regime; while nonlinear electrophoresis,

SUBMITTER: Vaghef-Koodehi A 

PROVIDER: S-EPMC10968109 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature