Rational Design of Key Enzymes to Efficiently Synthesize Phycocyanobilin in Escherichia coli.

ABSTRACT: Phycocyanobilin (PCB) is a natural blue tetrapyrrole chromophore that is found in phycocyanin and plays an essential role in photosynthesis. Due to PCB's antioxidation, anti-inflammatory and anti-cancer properties, it has been utilized in the food, pharmaceutical and cosmetic industries. Currently, the extraction of PCB from Spirulina involves complex processes, which has led to increasing interest in the biosynthesis of PCB in Escherichia coli. However, the PCB titer remains low because of the poor activity of key enzymes and the insufficient precursor supply. Here, the synthesis of PCB was firstly improved by screening the optimal heme oxygenase (HO) from Thermosynechococcus elongatus BP-1(HO^T) and PCB: ferredoxin oxidoreductase from Synechocystis s

SUBMITTER: Wang Z 

PROVIDER: S-EPMC10968380 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature