SP1 Gene Methylation in Head and Neck Squamous Cell Cancer in HPV-Negative Patients.

ABSTRACT: There is still much to learn about the epigenetic mechanisms controlling gene expression during carcinogenesis. When researching aberrant DNA methylation, active proliferative tumor cells from head and neck squamous cell cancer (HNSCC) can be used as a model. The aim of the study was to investigate the methylation status of CDKN1, CDKN2A, MYC, Smad3, SP1, and UBC genes in tumor tissue (control-normal tissue) in 50 patients (37 men and 13 women) with HPV-negative HNSCC. Methods: Bisulfite conversion methods and methyl-sensitive analysis of high-resolution melting curves were used to quantify the methylation of genes. In all patients and across various subgroups (tongue carcinoma, laryngeal and other types of carcinomas T2, T3, T4 status; age before

SUBMITTER: Jumaniyazova E 

PROVIDER: S-EPMC10970621 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature