Ontology highlight
ABSTRACT:
SUBMITTER: Smirnov K
PROVIDER: S-EPMC10971144 | biostudies-literature | 2024 Mar
REPOSITORIES: biostudies-literature
Smirnov Kirill K Weiss Florian F Hatzl Anna-Maria AM Rieder Lukas L Olesen Kjeld K Jensen Sanne S Glieder Anton A
Journal of fungi (Basel, Switzerland) 20240305 3
CRISPR (clustered regularly interspaced short palindromic repeats)-based technologies are powerful, programmable tools for site-directed genome modifications. After successful adaptation and efficient use of CRISPR-Cas9 for genome engineering in methylotrophic yeast <i>Komagataella phaffii</i>, a broader variety of employable endonucleases was desired to increase the experimental flexibility and to provide alternatives in case there are specific legal restrictions in industrial research due to t ...[more]