Evaluation of Antimicrobial Properties, Cell Viability, and Metalloproteinase Activity of Bioceramic Endodontic Materials Used in Vital Pulp Therapy.

ABSTRACT: This study aimed to evaluate the antimicrobial properties, cell viability, and matrix metalloproteinase (MMP) inhibition capacity of several endodontic materials aimed at vital pulp therapy: Pro Root MTA^®, EndoSequence^®, Biodentine^®, MTA Angelus^®, TheraCal LC^®, and BioC Repair^®. The materials were prepared according to the manufacturer's instructions. Antimicrobial tests were conducted using a microcosm biofilm model, cell viability was assessed using murine fibroblasts (L929), and MMP activity was analyzed through electrophoresis. The results showed that BioC Repair^®, Biodentine^®, and EndoSequence^® exhibited similar antimicrobial properties, while MTA Angelus^® and ProRoot MTA^® had inferior results but were comparable to each other. In terms of cell viability, no significant differences were observed among the materials. EndoSequence^® demonstrated the highest MMP inhibition capacity. In conclusion, BioC Repair^®, Biodentine^®, EndoSequence^®, and TheraCal^® showed better antimicrobial properties among the tested materials. The materials did not exhibit significant differences in terms of cytotoxicity. However, EndoSequence^® displayed superior MMP inhibition capacity.

SUBMITTER: Immich F 

PROVIDER: S-EPMC10971313 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature