A Molecular Hybrid of the GFP Chromophore and 2,2'-Bipyridine: An Accessible Sensor for Zn²⁺ Detection with Fluorescence Microscopy.

ABSTRACT: The few commercially available chemosensors and published probes for in vitro Zn²⁺ detection in two-photon microscopy are compromised by their flawed spectroscopic properties, causing issues in selectivity or challenging multistep syntheses. Herein, we present the development of an effective small molecular GFP chromophore-based fluorescent chemosensor with a 2,2'-bipyridine chelator moiety (GFZnP BIPY) for Zn²⁺ detection that has straightforward synthesis and uncompromised properties. Detailed experimental characterizations of the free and the zinc-bound compounds within the physiologically relevant pH range are presented. Excellent photophysical characteristics are reported, including a 53-fold fluorescence enhancement with excitation and emission maxima at 422 nm a

SUBMITTER: Csomos A 

PROVIDER: S-EPMC10971390 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature