In-Situ Metabolic Profiling of Different Kinds of <i>Rheum palmatum</i> L. by Laser Desorption-Dielectric Barrier Discharge Ionization Mass Spectrometry Imaging.

ABSTRACT: With its high resolving power and sensitivity, mass spectrometry is considered the most informative technique for metabolite qualitation and quantification in the plant sciences. However, the spatial location information, which is crucial for the exploration of plant physiological mechanisms, is lost. Mass spectrometry imaging (MSI) is able to visualize the spatial distribution of a large number of metabolites from the complex sample surface in a single experiment. In this paper, a flexible and low-cost laser desorption-dielectric barrier discharge ionization-MSI (LD-DBDI-MSI) platform was constructed by combining an LD system with an in-line DBDI source, a high-precision sample translation stage, and an ambient mass spectrometer. It can be operated at a spatial resolution of 20 μm in an a

SUBMITTER: Xiao X 

PROVIDER: S-EPMC10972210 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature