Mice, myeloid cells, and dengue: a new model for unraveling vascular leakage mysteries.

ABSTRACT:

Introduction

Severe dengue is thought to be caused by an excessive host immune response.

Methods

To study the pathogenesis of severe dengue, we developed a novel model using LysM Cre⁺Ifnar^flox/flox mice carrying depleted Ifnar expression only in subsets of murine myeloid cells.

Results

Although dengue virus (DENV) clinical isolates were not virulent in LysM Cre⁺Ifnar^flox/flox mice, mouse-adapted DV1-5P7Sp and DV3P12/08P4Bm, which were obtained by passaging the spleen or bone marrow of mice, demonstrated 100% lethality with severe vascular leakage in the liver and small intestine. DV1-5P7Sp and DV3P12/08P4Bm harbored five and seven amino acid substitutions, respectively. Infection also induced neutrophil

SUBMITTER: Kurosu T 

PROVIDER: S-EPMC10972876 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature