SAP-expressing T peripheral helper cells identify systemic lupus erythematosus patients with lupus nephritis.

ABSTRACT:

Introduction

T follicular (TFH) and peripheral helper (TPH) cells have been increasingly recognized as a pathogenic subset of CD4 T cells in systemic lupus erythematosus (SLE). The SLAM Associated Protein (SAP) regulates TFH and TPH function by binding to the co-stimulatory signaling lymphocyte activation molecule family (SLAMF) receptors that mediate T cell - B cell interactions. SAP and SLAMF are critical for TPH-dependent B cell maturation into autoantibody-producing plasma cells that characterize SLE pathogenesis. We hypothesized that SAP-expressing TPH cells are involved in the pathogenesis of lupus nephritis (LN).

Methods

Peripheral blood mononuclear cells (PBMC) were isolated using density gradient separation from whole blood. Cells were stained for cell surface marke

SUBMITTER: Gartshteyn Y 

PROVIDER: S-EPMC10972949 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature