Ultra high content analyses of circulating and tumor associated hybrid cells reveal phenotypic heterogeneity.

ABSTRACT: Persistently high, worldwide mortality from cancer highlights the unresolved challenges of disease surveillance and detection that impact survival. Development of a non-invasive, blood-based biomarker would transform survival from cancer. We demonstrate the functionality of ultra-high content analyses of a newly identified population of tumor cells that are hybrids between neoplastic and immune cells in patient matched tumor and peripheral blood specimens. Using oligonucleotide conjugated antibodies (Ab-oligo) permitting cyclic immunofluorescence (cyCIF), we present analyses of phenotypes among tumor and peripheral blood hybrid cells. Interestingly, the majority of circulating hybrid cell (CHC) subpopulations were not identified in tumor-associated hybrids. These results highlight the effi

SUBMITTER: Whalen RM 

PROVIDER: S-EPMC10973471 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature