Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples.

ABSTRACT: In 2015, we launched the mesoSPIM initiative, an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging larger samples at higher speed and resolution has increased, requiring major improvements in the capabilities of such microscopes. Here, we introduce the next-generation mesoSPIM ("Benchtop") with a significantly increased field of view, improved resolution, higher throughput, more affordable cost, and simpler assembly compared to the original version. We develop an optical method for testing detection objectives that enables us to select objectives optimal for light-sheet imaging with large-sensor cameras. The improved mesoSPIM achieves high spatial resolution (1.5 µm laterally, 3.3 µm axially) across the entire

SUBMITTER: Vladimirov N 

PROVIDER: S-EPMC10973490 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature