Monolayer culture alters EGFR inhibitor response through abrogation of microRNA-mediated feedback regulation.

ABSTRACT: Ex vivo drug screening is a potentially powerful tool for the future of cancer care, but the accuracy of results is contingent on the culture model. Both monolayer (2D) and spheroid (3D) culture systems offer advantages, but given the differences in mechanical environment, we hypothesized that that the suitability of one system over another would be critical for screening drugs with mechanical targets in mechanical tissues. HCC827 lung adenocarcinoma cells were challenged with EGFR tyrosine kinase inhibitors in monolayer and spheroid culture. RNA sequencing was performed on cells in both conditions to assess culture-induced transcriptional changes that could account for differences in drug response and differences in EGFR expression detected by immunostain. A microRNA microarray was perfor

SUBMITTER: Florio A 

PROVIDER: S-EPMC10973516 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature