Application of Liquid Chromatography Coupled to Mass Spectrometry for Direct Estimation of the Total Levels of Adenosine and Its Catabolites in Human Blood.

ABSTRACT: Adenosine is a multifunctional nucleoside with several roles across various levels in organisms. Beyond its intracellular involvement in cellular metabolism, extracellular adenosine potently influences both physiological and pathological processes. In relation to its blood level, adenosine impacts the cardiovascular system, such as heart beat rate and vasodilation. To exploit the adenosine levels in the blood, we employed the liquid chromatography method coupled with mass spectrometry (LC-MS). Immediately after collection, a blood sample mixed with acetonitrile solution that is either enriched with ¹³C-labeled adenosine or a newly generated mixture is transferred into the tubes containing the defined amount of ¹³C-labeled adenosine. The ¹³C-enriched isotopi

SUBMITTER: Sofranko J 

PROVIDER: S-EPMC10974345 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature