Positive Regulatory Roles of Manihot esculenta HAK5 under K⁺ Deficiency or High Salt Stress.

ABSTRACT: HAK/KUP/KT family members have been identified as playing key roles in K⁺ uptake and salt tolerance in numerous higher plants. However, their functions in cassava (Manihot esculenta Cantz) remain unknown. In this study, a gene encoding for a high-affinity potassium transporter (MeHAK5) was isolated from cassava and its function was investigated. Subcellular localization analysis showed that MeHAK5 is a plasma membrane-localized transporter. RT-PCR and RT-qPCR indicated that MeHAK5 is predominantly expressed in cassava roots, where it is upregulated by low potassium or high salt; in particular, its highest expression levels separately increased by 2.2 and 2.9 times after 50 µM KCl and 150 mM NaCl treatments. When heterologously expressed in yeast, M

SUBMITTER: Luo M 

PROVIDER: S-EPMC10974855 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature