Duplex Droplet Digital PCR Assay for Quantification of Hepatitis E Virus in Food.

ABSTRACT: Hepatitis E virus (HEV) represents an emerging risk in industrialized countries where the consumption of contaminated food plays a pivotal role. Quantitative real-time RT-PCR (RT-qPCR) is one of the most suitable methods for the detection and quantification of viruses in food. Nevertheless, quantification using RT-qPCR has limitations. Droplet digital PCR (ddPCR) provides the precise quantification of nucleic acids without the need for a standard curve and a reduction in the effect on virus quantification due to the presence of inhibitors. The objectives of the present work were (i) to develop a method for the absolute quantification of HEV in swine tissues based on ddPCR technology and provide internal process control for recovery assessment and (ii) to evaluate the performance of the met

SUBMITTER: La Bella G 

PROVIDER: S-EPMC10975721 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature