Calcium Chloride Treatment Enhances Antigen Production in Foot-and-Mouth Disease Vaccines for Serotypes SAT1 and SAT3.

ABSTRACT: Foot-and-mouth disease (FMD) is a highly contagious viral infection causing acute and severe vesicular lesions in cattle and pigs, which has prompted global vaccination policies. This study presents a technique for enhancing antigen yield in SAT1 BOT and SAT3 ZIM by treatment with calcium chloride (CaCl₂). We tested changes in cell viability in BHK-21 suspension cells treated with varying concentrations of CaCl₂. The optimal CaCl₂ concentration was determined based on antigen yield. The timing of CaCl₂ supplementation relative to FMD virus inoculation was tested. Finally, the optimal medium for antigen production was identified. We observed a concentration-dependent decrease in BHK-21 cell viability at >7.5 mM CaCl₂. A CaCl₂

SUBMITTER: Kim D 

PROVIDER: S-EPMC10976096 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature