Nucleosome Dynamics Derived at the Single-Molecule Level Bridges Its Structures and Functions.

ABSTRACT: Nucleosome, the building block of chromatin, plays pivotal roles in all DNA-related processes. While cryogenic-electron microscopy (cryo-EM) has significantly advanced our understanding of nucleosome structures, the emerging field of single-molecule force spectroscopy is illuminating their dynamic properties. This technique is crucial for revealing how nucleosome behavior is influenced by chaperones, remodelers, histone variants, and post-translational modifications, particularly in their folding and unfolding mechanisms under tension. Such insights are vital for deciphering the complex interplay in nucleosome assembly and structural regulation, highlighting the nucleosome's versatility in response to DNA activities. In this Perspective, we aim to consolidate the latest advancements in nuc

SUBMITTER: Chen P 

PROVIDER: S-EPMC10976579 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature