Decellularized human amniotic membrane scaffolds: influence on the biological behavior of dental pulp stem cells.

ABSTRACT:

Objective

The objective of this study was to assess the characterization of human acellular amniotic membrane (HAAM) using various decellularization methods and their impact on the proliferation and differentiation of human dental pulp stem cells (DPSCs). The goal was to identify scaffold materials that are better suited for pulp regeneration.

Methods

Six different decellularization methods were used to generate the amniotic membranes. The characteristics of these scaffolds were examined through hematoxylin and eosin (H&E) staining, scanning electron microscopy (SEM), and immunohistofluorescence staining (IHF). The DPSCs were isolated, cultured, and their capacity for multidirectional differentiation was verified. The third generation (P3) DPSCs, were then combined with HAAM

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC10976669 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature