Systematic immune cell dysregulation and molecular subtypes revealed by single-cell RNA-seq of subjects with type 1 diabetes.

ABSTRACT:

Background

Type 1 diabetes mellitus (T1DM) is a prototypic endocrine autoimmune disease resulting from an immune-mediated destruction of pancreatic insulin-secreting β  cells. A comprehensive immune cell phenotype evaluation in T1DM has not been performed thus far at the single-cell level.

Methods

In this cross-sectional analysis, we generated a single-cell transcriptomic dataset of peripheral blood mononuclear cells (PBMCs) from 46 manifest T1DM (stage 3) cases and 31 matched controls.

Results

We surprisingly detected profound alterations in circulatory immune cells (1784 dysregulated genes in 13 immune cell types), far exceeding the count in the comparator systemic autoimmune disease SLE. Genes upregulated in T1DM were involved in WNT signaling, interferon signaling

SUBMITTER: Honardoost MA 

PROVIDER: S-EPMC10976681 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature