Improved therapeutic consistency and efficacy of CD317⁺ MSCs through stabilizing TSG6 by PTX3.

ABSTRACT:

Background

Previously, we have demonstrated that the batch variations of human platelet lysate (conventional MSC expansion medium) induce MSC heterogeneity and therapeutic inconsistency. On the other hand, the MSCs expanded with chemical defined medium have improved therapeutic consistency.

Methods

In the current study, we studied the MSC subpopulation composition and variation in different types and batches of MSC expansion medium with scRNA-seq analysis.

Results

MSCs expanded with different batches of media have higher levels of heterogeneity from the perspective of cell subpopulation composition at transcriptome levels and therapeutic inconsistency. The CD317⁺ subpopulation has enhanced immune suppression activities. And the percentage of CD317⁺

SUBMITTER: Shi S 

PROVIDER: S-EPMC10976765 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature