Effects of nicotine on microRNA-124 expression in bile duct ligation-induced liver fibrosis in rats.

ABSTRACT:

Background

Nicotine, the main compound of smoking may exert its effects by changing the expression of microRNAs (miRNAs). This study was conducted to further investigate the molecular mechanisms of miRNA-dependent effects of nicotine in an animal model of liver fibrosis.

Methods

The bile duct ligation (BDL) approach was used to create a model of liver fibrosis. Twenty-four male Wistar rats were used in the study. The effects of nicotine administration on miRNA-124 expression, as well as alpha-smooth muscle actin (liver fibrosis marker) and chemokine ligand 2 (an inflammatory chemokine), were investigated using RT-qPCR. In addition, the mRNA and protein expression of signal transducer and activator of transcription 3 (STAT-3; as a potential target for miRNA-124) were investig

SUBMITTER: Hajiasgharzadeh K 

PROVIDER: S-EPMC10976767 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature