Stage-specific expression of the mitochondrial co-chaperonin of Leishmania donovani, CPN10.

ABSTRACT: BACKGROUND: Leishmania spp., in the course of their parasitic life cycle, encounter two vastly different environments: the gut of sandflies and the phagosomes of mammalian macrophages. During transmission into a mammal, the parasites are exposed to increased ambient temperature as well as to different carbon sources. Molecular chaperones or heat shock proteins are implicated in the necessary adaptations which involve the ordered differentiation from the flagellated, extracellular promastigote to the intracellular amastigote stage. RESULTS: Here, we show that the Leishmania donovani co-chaperonin, CPN10, is synthesised to a significantly increased concentration during in vitro differentiation to the amastigote stage. We show by fluorescence microscopy and by immunogold electron microscopy t

SUBMITTER: Zamora-Veyl FB 

PROVIDER: S-EPMC1097755 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature