Saliva sampling method influences oral microbiome composition and taxa distribution associated with oral diseases.

ABSTRACT: Saliva is a readily accessible and inexpensive biological specimen that enables investigation of the oral microbiome, which can serve as a biomarker of oral and systemic health. There are two routine approaches to collect saliva, stimulated and unstimulated; however, there is no consensus on how sampling method influences oral microbiome metrics. In this study, we analyzed paired saliva samples (unstimulated and stimulated) from 88 individuals, aged 7-18 years. Using 16S rRNA gene sequencing, we investigated the differences in bacterial microbiome composition between sample types and determined how sampling method affects the distribution of taxa associated with untreated dental caries and gingivitis. Our analyses indicated significant differences in microbiome composition between the samp

SUBMITTER: Roca C 

PROVIDER: S-EPMC10977688 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature