Transcriptome-wide analysis of the differences between MCF7 cells cultured in DMEM or αMEM.

ABSTRACT: MCF7 cells have been used as an experimental model for breast cancer for decades. Typically, a culture medium is designed to supply cells with the nutrients essential for their continuous proliferation. Each medium has a specific nutritional composition. Therefore, cells cultured in different media may exhibit differences in their metabolism. However, only a few studies have investigated the effects of media on cells. In this study, we compared the effects of Dulbecco's modified Eagle medium (DMEM) and minimum essential medium alpha modification (αMEM) on MCF7 cells. The two media differentially affected the morphology, cell cycle, and proliferation of MCF7 cells, but had no effect on cell death. Replacement of DMEM with αMEM led to a decrease in ATP production and an increase in reactive

SUBMITTER: Jiao Y 

PROVIDER: S-EPMC10977736 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature