Gsα Regulates Macrophage Foam Cell Formation During Atherosclerosis.

ABSTRACT:

Background

Many cardiovascular pathologies are induced by signaling through G-protein-coupled receptors via Gsα (G protein stimulatory α subunit) proteins. However, the specific cellular mechanisms that are driven by Gsα and contribute to the development of atherosclerosis remain unclear.

Methods

High-throughput screening involving data from single-cell and bulk sequencing were used to explore the expression of Gsα in atherosclerosis. The differentially expression and activity of Gsα were analyzed by immunofluorescence and cAMP measurements. Macrophage-specific Gsα knockout (Mac-Gsα^KO) mice were generated to study the effect on atherosclerosis. The role of Gsα was determined by transplanting bone marrow and performing assays for foam cell formation, Dil-ox-LDL (ox

SUBMITTER: Ma C 

PROVIDER: S-EPMC10978275 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature