CROPseq-multi: a universal solution for multiplexed perturbation in high-content pooled CRISPR screens.

ABSTRACT: Forward genetic screens seek to dissect complex biological systems by systematically perturbing genetic elements and observing the resulting phenotypes. While standard screening methodologies introduce individual perturbations, multiplexing perturbations improves the performance of single-target screens and enables combinatorial screens for the study of genetic interactions. Current tools for multiplexing perturbations are limited by technical challenges and do not offer compatibility across diverse screening methodologies, including enrichment, single-cell sequencing, and optical pooled screens. Here, we report the development of CROPseq-multi (CSM), a CROPseq¹-inspired lentiviral system to multiplex Streptococcus pyogenes (Sp) Cas9-based perturbations with versatile readout co

SUBMITTER: Walton RT 

PROVIDER: S-EPMC10979941 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature