Single-animal, single-tube RNA extraction for comparison of relative transcript levels via qRT-PCR in the tardigrade <i>Hypsibius exemplaris.</i>

ABSTRACT: The tardigrade Hypsibius exemplaris is an emerging model organism renowned for its ability to survive environmental extremes. To explore the molecular mechanisms and genetic basis of such extremotolerance, many studies rely on RNA-sequencing (RNA-seq), which can be performed on populations ranging from large cohorts to individual animals. Reverse Transcription Polymerase Chain Reaction (RT-PCR) and RNA interference (RNAi) are subsequently used to confirm RNA-seq findings and assess the genetic requirements for candidate genes, respectively. Such studies require an efficient, accurate, and affordable method for RNA extraction and measurement of relative transcript levels by quantitative RT-PCR (qRT-PCR). This work presents an efficient single-tardigrade, single-tube RNA extraction me

SUBMITTER: Kirk MJ 

PROVIDER: S-EPMC10979942 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature