<i>Eed</i> controls craniofacial osteoblast differentiation and mesenchymal proliferation from the neural crest.

ABSTRACT: The histone methyltransferase Polycomb repressive complex 2 (PRC2) is required for specification of the neural crest, and mis-regulation of neural crest development can cause severe congenital malformations. PRC2 is necessary for neural crest induction, but the embryonic, cellular, and molecular consequences of PRC2 activity after neural crest induction are incompletely understood. Here we show that Eed, a core subunit of PRC2, is required for craniofacial osteoblast differentiation and mesenchymal proliferation after induction of the neural crest. Integrating mouse genetics with single-cell RNA sequencing and epigenetic profiling, our results reveal that conditional knockout of Eed after neural crest cell induction causes severe craniofacial hypoplasia, impaired craniofacial

SUBMITTER: Casey-Clyde T 

PROVIDER: S-EPMC10979956 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature