A feedback-driven brain organoid platform enables automated maintenance and high-resolution neural activity monitoring.

ABSTRACT: The analysis of tissue cultures, particularly brain organoids, requires a sophisticated integration and coordination of multiple technologies for monitoring and measuring. We have developed an automated research platform enabling independent devices to achieve collaborative objectives for feedback-driven cell culture studies. Our approach enables continuous, communicative, non-invasive interactions within an Internet of Things (IoT) architecture among various sensing and actuation devices, achieving precisely timed control of in vitro biological experiments. The framework integrates microfluidics, electrophysiology, and imaging devices to maintain cerebral cortex organoids while measuring their neuronal activity. The organoids are cultured in custom, 3D-printed chambers affixed to c

SUBMITTER: Voitiuk K 

PROVIDER: S-EPMC10979982 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature