An immune-competent human gut microphysiological system enables inflammation-modulation by Faecalibacterium prausnitzii.

ABSTRACT: Crosstalk of microbes with human gut epithelia and immune cells is crucial for gut health. However, there is no existing system for a long-term co-culture of human innate immune cells with epithelium and oxygen-intolerant commensal microbes, hindering the understanding of microbe-immune interactions in a controlled manner. Here, we established a gut epithelium-microbe-immune (GuMI) microphysiological system to maintain the long-term continuous co-culture of Faecalibacterium prausnitzii/Faecalibacterium duncaniae with colonic epithelium, antigen-presenting cells (APCs, herein dendritic cells and macrophages), and CD4⁺ naive T cells circulating underneath the colonic epithelium. In GuMI-APC condition, multiplex cytokine assays suggested that APCs contribute to the elevated level o

SUBMITTER: Zhang J 

PROVIDER: S-EPMC10980819 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature