In vivo evolution of antimicrobial resistance in a biofilm model of Pseudomonas aeruginosa lung infection.

ABSTRACT: The evolution of antimicrobial resistance (AMR) in biofilms has been repeatedly studied by experimental evolution in vitro, but rarely in vivo. The complex microenvironment at the infection site imposes selective pressures on the bacterial biofilms, potentially influencing the development of AMR. We report here the development of AMR in an in vivo mouse model of Pseudomonas aeruginosa biofilm lung infection. The P. aeruginosa embedded in seaweed alginate beads underwent four successive lung infection passages with or without ciprofloxacin (CIP) exposure. The development of CIP resistance was assessed at each passage by population analysis of the bacterial populations recovered from the lungs of CIP-treated and control mice, with subsequent whole-genome sequencing of selected isolates. As i

SUBMITTER: Higazy D 

PROVIDER: S-EPMC10980832 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature