CRISPR/Cpf1-FOKI-induced gene editing in Gluconobacter oxydans.

ABSTRACT: Gluconobacter oxydans is an important Gram-negative industrial microorganism that produces vitamin C and other products due to its efficient membrane-bound dehydrogenase system. Its incomplete oxidation system has many crucial industrial applications. However, it also leads to slow growth and low biomass, requiring further metabolic modification for balancing the cell growth and incomplete oxidation process. As a non-model strain, G. oxydans lacks efficient genome editing tools and cannot perform rapid multi-gene editing and complex metabolic network regulation. In the last 15 years, our laboratory attempted to deploy multiple CRISPR/Cas systems in different G. oxydans strains and found none of them as functional. In this study, Cpf1-based or dCpf1-based CRISPRi was co

SUBMITTER: Wang X 

PROVIDER: S-EPMC10980938 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature