Epac2 activation mediates glucagon-induced glucogenesis in primary rat hepatocytes.

ABSTRACT:

Aims/introduction

Glucagon plays an essential role in hepatic glucogenesis by enhancing glycogen breakdown, inducing gluconeogenesis, and suppressing glycogenesis. Moreover, glucagon increases cyclic adenosine monophosphate (cAMP) levels, thereby activating protein kinase A (PKA) and cAMP guanine nucleotide exchange factor (also known as Epac). Although the function of PKA in the liver has been studied extensively, the function of hepatic Epac is poorly understood. The aim of this study was to elucidate the role of Epac in mediating the action of glucagon on the hepatocytes.

Materials and methods

Epac mRNA and protein expression, localization, and activity in the hepatocytes were analyzed by reverse transcription polymerase chain reaction, western blotting, immunofluorescenc

SUBMITTER: Shiozaki-Takagi Y 

PROVIDER: S-EPMC10981141 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature